Ajax have been banned from bringing any fans to Stamford Bridge for their Champions League clash with Chelsea on November 5.
The ban comes following a suspended sentence imposed after Ajax's Champions League game against Benfica last season.
The Dutch champions have also been fined £43,000 and given a 'conditional penalty of 1 year for selling tickets at an away match', according to a statement on the club's website.
"UEFA has ruled on the events with Ajax supporters at Valencia-Ajax and the suspended sentence imposed after Benfica-Ajax last season. The biggest sanction is that Chelsea - Ajax will be played on November 5 (9 p.m.) without fans.
“Ajax has requested additional information from UEFA. Then it is checked whether an appeal is lodged," the club confirmed.
Ajax were previously fined €68,000 for two infractions on and off the field, including 'acts of damages and crowd disturbances' in the terraces and 'improper conduct' for their behaviour on the turf, in which six players received yellow cards in the 3-0 win over Valencia at the Mestalla.
Chelsea are level on points with Valencia in Group H, and Frank Lampard’s men will be targeting a win so as to get level with group leaders Ajax. Ligue 1 side Lille are yet to pick up a point as they host Valencia at home.